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目的:探讨宫颈癌患者外周血中CD4~+CD25~(+high)调节性T(regulator T cells,Tr)的表达及意义。方法:采用流式细胞术检测52例宫颈癌患者,35例健康女性外周血中CD4~+CD25~(+high)Tr、细胞毒性T细胞(cytotoxic T lymphocytes,CTL)和NK细胞,采用ELISA检测血清中-干扰素(interferon,IFN-)的表达水平。结果:宫颈癌患者外周血CD4~+CD25~(+high)Tr占CD4~+T淋巴细胞的百分比为(7.18±2.32)%,高于健康女性组(P<0.05);宫颈癌患者外周血CD4~+CD25~(+high)Tr水平与CTL、NK细胞及IFN-水平呈负相关。结论:宫颈癌患者外周血中具免疫抑制活性的CD4~+CD25~(+high)Tr水平较高,参与宫颈癌患者的肿瘤免疫抑制。
Objective: To investigate the expression and significance of CD4 ~ + CD25 ~ (+ high) regulatory T cells (Trp) in peripheral blood of patients with cervical cancer. Methods: Flow cytometry was used to detect CD4 ~ + CD25 ~ (+ high) Tr, cytotoxic T lymphocytes (CTL) and NK cells in peripheral blood of 52 cases of cervical cancer and 35 healthy women. Serum levels of interferon (interferon, IFN-) expression levels. Results: The percentage of CD4 ~ + CD25 ~ (+ high) Tr in peripheral blood of patients with cervical cancer was (7.18 ± 2.32)% higher than that of healthy women (P <0.05) CD4 ~ + CD25 ~ (+ high) Tr levels were negatively correlated with CTL, NK cells and IFN-γ. Conclusion: The level of CD4 ~ + CD25 ~ (+ high) Tr with immunosuppressive activity in peripheral blood of patients with cervical cancer is higher, which is involved in tumor immunosuppression in patients with cervical cancer.