目的研究临床分离致病菌抗药基因情况及其对消毒剂的抗性水平。方法采用基因扩增法和肉汤稀释法分别检测了三种21株革兰阴性杆菌的耐消毒剂基因和苯扎溴铵最低抑菌浓度及最低杀菌浓度。结果临床分离的铜绿假单胞菌、大肠杆菌和鲍曼不动杆菌等21株三种革兰阴性杆菌,其中有13株携带耐消毒剂基因,携带率为61.9%。7株铜绿假单胞菌对苯扎溴铵M IC均低于标准菌株,共有7株菌的MBC高于标准菌株。结论临床分离的三种21株革兰阴性杆菌多数携带耐消毒剂基因,有33.3%的菌株对苯扎溴铵的MBC高于标准株,提示其抗力有所增强。 Objective To study the clinical isolates of pathogenic bacteria and their resistance to disinfectants. Methods The antibacterial agents of three strains of 21 gram-negative bacilli and the minimum inhibitory concentrations and minimum bactericidal concentrations of benzalkonium bromide were detected by gene amplification and broth dilution respectively. Results Twenty-one strains of three gram-negative bacilli, Pseudomonas aeruginosa, Escherichia coli and Acinetobacter baumannii, were isolated clinically. Among them, 13 strains were resistant to disinfectant and their carriage rate was 61.9%. The M IC of 7 strains of Pseudomonas aeruginosa to benzalkonium bromide were lower than those of the standard strains, and the MBC of 7 strains were higher than the standard strains. Conclusion Most of the 21 strains of Gram-negative bacilli isolated from clinical isolates carry the disinfectant-resistant gene. The MBC of 33.3% isolates of benzalkonium bromide is higher than that of the standard strain, suggesting that its resistance is enhanced.