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目的探讨白首乌总苷与环磷酰胺(CY)合用对荷瘤小鼠免疫功能的影响。方法选择小鼠H22肝癌、S180腹水瘤制备肿瘤模型;按计划给药10 d后,称重计算胸腺指数,MTT比色法检查脾脏T、B淋巴细胞的增殖能力。结果白首乌总苷单独使用无明显抗肿瘤作用,但可增加荷瘤小鼠的胸腺指数(和模型组比较P<0.05)及T、B淋巴细胞的增殖能力(和模型组比较P<0.001)。白首乌总苷与CY联合使用,可明显增加CY治疗的荷H22小鼠胸腺指数(和CY组比较P<0.05)及T、B淋巴细胞增殖能力(和CY组比较P<0.001);可明显增加CY治疗的荷S180小鼠的体重及胸腺指数(和CY治疗组比较P<0.05),明显增强其B淋巴细胞增殖能力(和CY组比较P<0.01)。结论通过本实验证明白首乌总苷与常用化疗药CY合用可改善化疗后小鼠细胞免疫和体液免疫的功能,对常用化疗药有一定减毒作用。
Objective To investigate the effect of combined use of total glucosides of Cynanchum Polygonum with cyclophosphamide (CY) on immune function in tumor-bearing mice. Methods Tumor models were prepared with mouse H22 hepatocellular carcinoma and S180 ascites tumors. After 10 days of scheduled administration, thymus index was calculated and MTT colorimetric assay was used to examine the proliferation of T and B lymphocytes. Results The total glucosides of P. bracteatum alone had no obvious anti-tumor effect, but it could increase the thymus index (P<0.05 compared with the model group) and the proliferative capacity of T and B lymphocytes in the tumor-bearing mice (P<0.001 compared with the model group. ). The combined use of total glucosides of P. sibiricum and CY significantly increased the thymus index (P<0.05 compared with CY group) and T, B lymphocyte proliferation ability of CHO-treated H22 mice (P<0.001 compared with CY group); Significantly increased body weight and thymus index of CY-treated S180 mice (P<0.05 compared with CY treated group) significantly enhanced the proliferation of B lymphocytes (P<0.01 compared with CY group). Conclusion This experiment demonstrates that the combined use of total glucosides of P. bracteatum with commonly used chemotherapeutic drugs CY can improve the function of cellular immunity and humoral immunity in mice after chemotherapy, and has a certain attenuating effect on commonly used chemotherapy drugs.