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绪言由于开始用野生果树作为将来育种的材料,就需要将大量的品种和品系在果园里长期栽培保存,这样在经济上和劳力上都是浪费的。为此,研究关于遗传种质,特别是接穗长期冻结保存的技术,无论在日本和世界各国都是迫切需要的。对苹果(旭)的枝条在液态氮中的冷却保存与加温解冻方法和予冻温度进行了研究。其结果,先预冻到-40℃后,放入液态氮中,再在0℃的空气中融解,效果最好。并且,予冻到-40℃后,把冷却到液态氮温度的新梢上的芽进行芽接,成活率约80%,成活的芽大部分
INTRODUCTION Since wild fruit trees have been used as materials for future breeding, a large number of varieties and strains need to be cultivated for long periods in the orchards, which is economically and laboriously wasteful. For this reason, research on the technology of long-term freeze-preserved genetic germplasm, especially scion, is urgently needed both in Japan and in all countries in the world. The method of cooling, thawing and thawing of apple (Asahi) branches in liquid nitrogen was studied. As a result, after pre-freezing to -40 ° C, it is put into liquid nitrogen and melted in air at 0 ° C to obtain the best effect. And, to be frozen to -40 ° C, the cooling to the liquid nitrogen temperature on the bud shoot budding, the survival rate of about 80%, most of the surviving bud