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目的建立HPLC法同时测定拟缺香茶菜不同入药部位中布卢门醇A、尾叶香茶菜丙素、鄂西香茶菜素含量的方法。方法色谱柱:YMC C18(4.6 mm×250 mm,5μm)反相色谱柱;流动相:乙腈-水(32∶68)等度洗脱;流速:1.0 m L/min;柱温:30℃;检测波长:230 nm。结果布卢门醇A、尾叶香茶菜丙素、鄂西香茶菜素分别在0.001 1~0.022 2 g/L(r=0.999 7),0.001 9~0.037 1g/L(r=0.999 5),0.013 3~0.265 g/L(r=0.999 7)范围内呈良好的线性关系;平均回收率分别为99.58%(RSD=2.29%),99.51%(RSD=1.65%),102.78%(RSD=1.57%)。不同产地拟缺香茶菜不同部位中3个活性成分的含量均以叶中最高。结论所建立的方法灵敏、准确、稳定,可用于拟缺香茶菜不同部位中3个活性成分的含量测定。
OBJECTIVE To establish a HPLC method for the simultaneous determination of Blumemethanol A, Phyllostachys praecox, Betacaceae in different medicinal parts of Xiang tea. Methods Column: YMC C18 (4.6 mm × 250 mm, 5 μm) reversed-phase column was used. The mobile phase consisted of isocratic elution of acetonitrile-water (32:68). The flow rate was 1.0 m L / Detection wavelength: 230 nm. Results The contents of Blumemethanol A, Aloe Vera and Acacia were 0.001 ~ 0.022 2 g / L (r = 0.999 7) and 0.001 9 ~ 0.037 1 g / L (r = 0.999 5 (RSD = 1.29%), 99.51% (RSD = 1.65%) and 102.78% (RSD), respectively. The average recoveries were 99.58% = 1.57%). The content of the three active ingredients in different parts of Xiangchacha from different areas were the highest in the leaves. Conclusion The established method is sensitive, accurate and stable and can be used to determine the content of three active ingredients in different parts of Xiang tea.