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目前在检测细胞上清液中低含量的IL-2时,仍然用IL-2依赖性杀伤性T细胞系(CTLL)做生物学试验。虽然此方法敏感度较高,甚至可测出8pg/ml的IL-2含量,但也存在许多缺点,如需要较长的检测周期,需要培养靶细胞,此外支原体污染和细胞毒因子可掩盖IL-2的活性,而残留在粗提液中的分裂原对某些靶细胞有类似IL-2的作用。现在正在发展的酶联或放射性免疫检测都面临一个重要的困难,即其敏感度大大低于生物活性检测法。为此作者设计了一种简单、方便、并可去除其它因素影响的连
At present, IL-2-dependent killer T cell line (CTLL) is still used for biological tests when detecting low levels of IL-2 in cell supernatants. Although this method is more sensitive and can even detect IL-2 levels of 8 pg / ml, there are a number of drawbacks, such as the need for longer detection cycles and the need for culturing of target cells. In addition, mycoplasma contamination and cytotoxic agents can mask IL -2 activity, whereas the split remaining in the crude extract acts like IL-2 on some target cells. Enzyme-linked or radioimmunoassay now under development is facing an important difficulty, its sensitivity is significantly lower than the biological activity detection method. For this reason, the author designed a simple, convenient, and can remove the influence of other factors