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应用免疫胶金技术证实钩端螺旋体脂多糖（Ｌ－ＬＰＳ）作用于体外培养的小鼠成纤维细胞后５ｍｉｎ即能侵入胞浆，３０ｍｉｎ后可在细胞核中检出Ｌ．一！Ｉ５。钩端螺旋体实验感染的豚鼠各内脏组织的细胞间隙、胞浆及核等内均存在１．一！，ＰＳ，尤以肾和肺脏检出阳性率较高。Ｌ－ＬＰＳ检测阳性的小鼠成纤维细胞和感染豚鼠内脏组织细胞均出现相似的超微结构病变，如内质网扩张、膜旁核糖体脱落、线粒体嵴或外膜消失等。实验结果表明Ｌ－ＬＰＳ是钩端螺旋体主要致病物质之一。
Immunocytochemistry showed that L-LPS could invade the cytoplasm 5 minutes after cultured in vitro, and could be detected in the nucleus after 30 minutes. one! I5. The intercellular space, cytoplasm and nucleus of visceral tissues of guinea pig infected with Leptospira were all present in the experiment1. one! , PS, especially in the positive detection rate of kidney and lung higher. L-LPS-positive mouse fibroblasts and infected guinea pig visceral tissue cells showed similar ultrastructural lesions, such as endoplasmic reticulum dilatation, parafascicular ribosome shedding, mitochondrial ridges or adventitia disappeared. The experimental results show that L-LPS is one of the main pathogens of Leptospira.