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目的：研究乙型肝炎病毒（ＨＢＶ）感染者外周血单个核细胞（ＰＢＭＣｓ）中ＨＢＶＤＮＡ的存在及其与血清ＨＢＶＤＮＡ的关系。方法：用ＰＣＲ法及斑点杂交法对５０例ＨＢｓＡｇ（＋）的感染者ＰＢＭＣｓＨＢＶＤＮＡ进行检测。同时用ＰＣＲ法检测感染者血清ＨＢＶＤＮＡ。结果：ＰＣＲ法和斑点杂交法分别从３５份和３２份ＰＢＭＣｓ检出ＨＢＶＤＮＡ，阳性率分别为７０．０％和６４．０％，各种临床型乙型肝炎ＰＢＭＣｓＨＢＶＤＮＡ检出率均为６０．０％左右。ＰＣＲ法检测血清ＨＢＶＤＮＡ阳性率为３６．０％。结论：ＨＢＶ存在于ＨＢＶ感染者ＰＢＭＣｓ中，并且ＨＢＶ可单独存在于感染者ＰＢＭ－Ｃｓ，ＨＢＶ感染者各种临床型肝炎ＰＢＭＣｓＨＢＶＤＮＡ检出率无明显差异
Objective: To investigate the presence of HBVDNA in peripheral blood mononuclear cells (PBMCs) from patients with hepatitis B virus infection and its relationship with serum HBVDNA. Methods: HBV DNA of 50 PBMCs infected with HBsAg (+) were detected by PCR and dot blot hybridization. At the same time using PCR method to detect serum HBV DNA. Results: HBVDNA was detected by PCR and dot blot hybridization in 35 and 32 PBMCs respectively, the positive rates were 70.0% and 64.0%, respectively, and the detection rates of HBVDNA in all kinds of clinical hepatitis B were 60.0 %about. The positive rate of serum HBVDNA by PCR was 36.0%. CONCLUSION: HBV is present in PBMCs of HBV-infected persons, and HBV can exist alone in infected PBM-Cs. There is no significant difference in the detection rate of HBVDNA among PBMCs of various clinical hepatitis patients with HBV infection