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目的通过质子磁共振波谱分析(magneticresonancespectroscopy,MRS)单次大剂量照射后脑照射区域内的代谢和超微结构变化,为放射性脑损伤发生机理的研究提供分子生物学水平上的实验研究资料和理论基础。方法将40只家兔随机分为4组,分别给予单次大剂量10、15、20Gy和30Gy照射,建立放射性脑损伤家兔实验动物模型,通过测量照射前后动物乳酸(Lacticacid,Lac)、N-乙酰天门冬氨酸(N-acetylaspartate,NAA)、胆碱(Choline,Cho)、肌酸(Creatine,Cr)等的波峰变化及照射不同时期内受照区域脑组织超微结构变化情况,比较照射前后不同时期各波峰变化情况并行统计学分析。结果在放射性脑损伤发生早期,质子MRS即有轻度降低,至电镜下出现明显的神经细胞水肿坏死时,质子MRS波谱分析已经有明显统计学意义的变化。结论质子MRS分析可以较好地反映超微结构的变化。
OBJECTIVE: To provide experimental data and theoretical basis for the study of the pathogenesis of radiation-induced brain injury through the study of the metabolism and ultrastructure of brain irradiated area after a single high-dose irradiation of magnetic resonance magnetic resonance spectroscopy (MRS) . Methods Forty rabbits were randomly divided into four groups. One dose of 10, 15, 20 Gy and 30 Gy were given respectively to establish the experimental animal model of radiation brain injury in rabbits. Lactic acid (Lac), N (NAA), Choline (Cho), Creatine (Cr) and so on, and the changes of ultrastructure of brain tissue in irradiated area at different periods of irradiation were compared Statistical analysis of the variation of each peak at different periods before and after irradiation. Results In the early stage of radioactive brain injury, the proton MRS decreased slightly. When electron microscopy showed obvious neuronal cell edema and necrosis, there was a statistically significant change in MRS spectra. Conclusions Proton MRS analysis can better reflect the changes of ultrastructure.