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目的 探讨白细胞提取物抗肺炎、肺纤维化的效果及其作用机制。方法 用博来霉素(BLM A6 ) 6mg/kg气管内注入 ,诱生小鼠肺炎和肺纤维化 ;预防治疗组肌内注射白细胞提取物 ,每日5 0 0 μg/kg;阳性对照组肌内注射丹参提取物 (IH76 4 3) ,每日 2 0mg/kg ,对照组肌内注射等体积的生理盐水。 30d后处死动物 ,用电镜观察其病理改变。结果 预防治疗组和阳性对照组经处理后 ,分别有6 0 %和 87%的动物肺组织炎症和纤维化得到改善。电镜观察显示 ,两组大多数动物肺胶原形成细胞数量、炎性细胞渗出、胶原纤维和弹力纤维都较阴性对照组明显减少 ,肺泡毛细血管形态较正常 ,肺泡隔内胶原纤维明显减少 ,保留了肺的蜂窝状结构。结论 白细胞提取物对博来霉素诱致的肺炎、肺纤维化具有一定的防治效果 ,其作用机制可能是通过调节机体的非特异性免疫功能而起作用
Objective To investigate the effect and mechanism of leukocyte extract against pneumonia and pulmonary fibrosis. Methods Bleomycin (BLM A6) 6mg / kg intratracheal injection induced pneumonia and pulmonary fibrosis in mice; prophylactic treatment group intramuscular injection of leukocyte extract daily 500 μg / kg; positive control group of muscle Salvia miltiorrhiza extract (IH76 4 3) was injected intraperitoneally at a dose of 20 mg / kg, and the control group was intramuscularly injected with an equal volume of normal saline. Animals were sacrificed after 30 days and their pathological changes were observed with electron microscope. Results After treatment, 60% and 87% of animals, respectively, had lung tissue inflammation and fibrosis improved after treatment. Electron microscopy showed that the number of pulmonary collagen forming cells, exudative inflammatory cells, collagen fibers and elastic fibers in most of the two groups were significantly decreased compared with the negative control group, alveolar capillary morphology was normal, collagen fibers in the alveolar septum significantly reduced, retained The lung’s honeycomb structure. Conclusion Leukocyte extract may have some preventive and therapeutic effects on bleomycin-induced pneumonia and pulmonary fibrosis. The mechanism may be through regulating the non-specific immune function of the body