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目的 :进一步揭示乙型肝炎患者免疫发病机理。方法 :采用夹心ELISA法检测血清IL 6、IL 8水平并用PCR法测HBV DNA ,自动电泳仪测γ 球蛋白。结果 :急性黄疸型、慢性活动型、重症型乙肝患者IL 6、IL 8均明显高于对照组 ;IL 6在三型间均有明显差异 ;IL 8重症型明显高于急、慢型 ;IL 6、IL 8与ALT呈明显正相关 ;HBeAg(+)组、HBV -DNA(+)组与相应阴性组比较IL 6水平明显升高 ,转阴后则IL 6、IL 8明显下降 ;IL 6与IL 8呈明显正相关。结论 :IL 6、IL 8参与乙型肝炎患者的免疫病理损伤 ,其水平可反映肝细胞受损程度 ,并可作为判定炎症活动程度和疗效的指标。
Objective: To further reveal the immune pathogenesis of hepatitis B patients. Methods: Serum IL 6 and IL 8 levels were measured by sandwich ELISA. HBV DNA was detected by PCR and γ-globulin was detected by auto-electrophoresis. Results: The levels of IL 6 and IL 8 in patients with acute jaundice, chronic active and severe hepatitis B were significantly higher than those in the control group. IL 6 was significantly different among the three types. IL 8 severe type was significantly higher than acute and chronic type. IL 6, IL 8 and ALT was positively correlated; IL 6 levels in HBeAg (+) group and HBV-DNA (+) group were significantly higher than that in the corresponding negative group, IL 6 and IL 8 decreased significantly There was a clear positive correlation with IL 8. CONCLUSION: IL 6 and IL 8 are involved in the immunopathological damage of hepatitis B patients. The level of IL 6 and IL 8 may reflect the degree of hepatocellular damage and may be used as an index to determine the degree of inflammatory activity and therapeutic effect.