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目的 探讨NF κB信号途径在新生儿败血症中的作用 ,为临床寻求以NF κB为靶点的治疗手段提供实验依据。方法 应用出生 1 0d新生大鼠及金黄色葡萄球菌制作新生鼠败血症模型。采用电泳迁移率改变分析 (EMSA)方法检测败血症新生鼠肝脏NF κB活性的表达情况和应用PDTC后肝脏NF κB活性变化情况。结果 金黄色葡萄球菌败血症新生鼠的肝脏NF κB在 1h开始有激活 ,4h达高峰。应用抗氧化剂PDTC对肝脏NF κB活化有抑制作用 ,且剂量越大 ,抑制作用越强。结论 (1 )新生鼠金黄色葡萄球菌败血症时肝脏NF κB有明显激活 ,且存在一高峰期。 (2 )抗氧化剂PDTC能对金黄色葡萄球菌败血症新生鼠肝脏NF κB活化有所抑制 ,且存在量 效依赖关系
Objective To investigate the role of NF-κB signaling in neonatal sepsis and provide experimental evidence for the clinical treatment of NF κB. Methods Neonatal rat sepsis model was made using born newborn rats and Staphylococcus aureus. Electrophoretic mobility shift assay (EMSA) was used to detect the expression of hepatic NF κB in liver of newborn rats with septicemia and the change of liver NF κB activity after application of PDTC. Results The NF κB activity in the liver of neonatal rats with Staphylococcus aureus sepsis was activated at 1h and peaked at 4h. Application of antioxidant PDTC on the liver NFκB activation inhibited, and the greater the dose, the stronger the inhibitory effect. Conclusion (1) Newborn rat Staphylococcus aureus sepsis hepatic NF κB activation, and there is a peak. (2) Antioxidant PDTC could inhibit the activation of NF κB in the liver of newborn rats with Staphylococcus aureus sepsis, and there was a dose-dependent relationship