论文部分内容阅读
目的:重组腺病毒Ad5-ADRbeta2-EGFP转染骨髓间充质干细胞(BMSCs)并植入心衰大鼠心肌,观察其心功能的变化。方法:采用部分缩窄腹主动脉法制备大鼠压力超负荷性慢性心力衰竭模型。分离和培养成年大鼠BMSCs,待细胞传代到第6代,用重组腺病毒Ad5-ADRbeta2-EGFP转染BMSCs48h后将BMSCs移植入心衰大鼠心肌内。实验共分3组:心衰组、移植BMSCs组及移植转染Ad5-ADRbeta2-EGFP的BMSCs组。移植BMSCs(1×104)4周后,检测大鼠血流动力学各指标的变化。结果:荧光显微镜下可见转染BMSCs绿色荧光蛋白表达达到80%以上。移植BMSCs组和移植转染Ad5-ADRbeta2-EGFP的BMSCs组的左心室舒张末压力(LVEDP)低于心衰组,左心室内压变化最大速率(±dp/dtmax)和心率均高于心衰组,差异均有统计学意义(P<0.05)。移植转染Ad5-ADRbeta2-EGFP的BMSCs组的左心室内压上升最大速率(+dp/dt)高于移植BMSCs组,差异有统计学意义(P<0.05)。结论:重组腺病毒Ad5-ADRbeta2-EGFP转染BMSCs并移植入心衰大鼠后,能够改善心衰大鼠的心功能。
OBJECTIVE: The recombinant adenovirus Ad5-ADRbeta2-EGFP was transfected into bone marrow-derived mesenchymal stem cells (BMSCs) and implanted into cardiac muscle of heart failure rats to observe the changes of cardiac function. Methods: Partial pressure narrowing of the abdominal aorta was used to prepare a rat model of stress overload chronic heart failure. The adult rat BMSCs were isolated and cultured. After the cells were passaged to the sixth passage, BMSCs were transplanted into the myocardium of the heart failure rats 48 hours after the BMSCs were transfected with the recombinant adenovirus Ad5-ADRbeta2-EGFP. The experiment was divided into 3 groups: heart failure group, BMSCs transplanted group and BMSCs transplanted with Ad5-ADRbeta2-EGFP group. After transplanted BMSCs (1 × 104) for 4 weeks, the indexes of hemodynamics in rats were detected. Results: Under the fluorescence microscope, the expression of green fluorescent protein in transfected BMSCs reached more than 80%. Left ventricular end-diastolic pressure (LVEDP) was lower in BMSCs-transplanted and BMSCs transfected with Ad5-ADRbeta2-EGFP than in HF, and the maximum rate of left ventricular pressure (± dp / dtmax) and heart rate Group, the difference was statistically significant (P <0.05). The maximum rate of increase of left ventricular pressure (+ dp / dt) in BMSCs transfected with Ad5-ADRbeta2-EGFP was significantly higher than that in BMSCs group (P <0.05). Conclusion: Transfection of recombinant adenovirus Ad5-ADRbeta2-EGFP into BMSCs and transplantation into heart failure rats can improve heart function of heart failure rats.