论文部分内容阅读
目的 探讨钙通道阻滞剂维拉帕米对兔耳增生性瘢痕的作用。方法 建立兔耳外伤性皮肤早期瘢痕增生模型 ,将 2 4只兔随机平均分为 3组 ,每组 8只 ,各组瘢痕内分别注射维拉帕米 2 5 μl(0 .0 6 2 5mg) 瘢痕、曲安缩松 2 5 μl(1mg 瘢痕 )、生理盐水 2 5 μl(0 .2 2 5mg 瘢痕 ) ,1次 10d ,共 2次。观察瘢痕外形变化。治疗后 2 0d在瘢痕处取材 ,切片行HE及Masson染色。光镜下观察 ,计算瘢痕增生指数、切片内成纤维细胞数密度 ,计算机辅助图象分析测算切片内胶原纤维面密度。结果 与生理盐水组相比 ,维拉帕米组和曲安缩松组瘢痕均变软、变平 ,色泽变浅 ,瘢痕增生指数及胶原纤维面密度均降低 ,3组间成纤维细胞数密度无显著变化 ;维拉帕米组瘢痕增生指数高于曲安缩松组 ,胶原纤维面密度两组间无显著性差异。结论 外伤性兔耳早期增生性瘢痕局部注射维拉帕米可降低瘢痕内胶原含量 ,引起瘢痕萎缩。本实验条件下维拉帕米的促瘢痕萎缩作用弱于曲安缩松。
Objective To investigate the effect of calcium channel blocker verapamil on hypertrophic scars in rabbit ears. Methods Rabbits ear traumatic skin scarring model was established. 24 rabbits were randomly divided into 3 groups with 8 rabbits in each group. Verapamil 25 μl (0.0625 mg) Scar, 25 mg of triamcinolone acetonide (1 mg scar), 25 μl normal saline (0.225 mg scar), 1 time for 10 days, 2 times in total. Observation of scar shape changes. Twenty days after treatment, the scar was harvested and stained with HE and Masson. Under light microscope, the scar hyperplasia index and the number density of fibroblasts in the slice were calculated. The surface area of collagen fibers in the slice was calculated by computer aided image analysis. Results Compared with the saline group, the scars in verapamil and triamcinolone acetonide groups were both soft and flattened, the color became lighter, the scar hyperplasia index and the collagen fiber density were decreased. The number density of fibroblasts No significant change; verapamil group scar hyperplasia index was higher than triamcinolone acetonide, collagen fiber density between the two groups no significant difference. Conclusion Local injection of verapamil into hypertrophic scars of traumatic rabbit ear can reduce collagen content in the scar and cause scar shrinkage. Verapamil under the experimental conditions to promote scar shrinkage is weaker than triamcinolone.