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惠兰(Cymbidium faberi)是中国兰属代表种之一,具有很高的观赏价值和经济价值,对其内生细菌进行研究不仅可以丰富植物内生细菌资源,还可以为探讨兰花与微生物之间的相互作用关系提供基础数据。本研究采用分离培养方法及16S r RNA基因序列测定对天目山野生蕙兰、在温室培养1年后的蕙兰根内生细菌遗传多样性进行了研究。结果表明:从野生蕙兰根内分离得到的97株细菌分属于变形菌门的α-变形菌纲、β-变形菌纲、γ-变形菌纲及厚壁菌门的13个属,其最优势类群为γ-变形菌纲(86.60%),Lelliottia(26.80%)为最优势菌属。从温室盆栽蕙兰根内分离得到的52株细菌分属于变形菌门的α-变形菌纲、β-变形菌纲、γ-变形菌纲及放线菌门的9个属,优势类群为β-变形菌纲(48.08%),优势菌属为草螺菌属(Herbaspirillum)(34.62%),其中菌株eh R17为潜在的新种。这些结果表明天目山野生蕙兰可培养根内生细菌多样性较其在温室培养1年后更为丰富,同时也说明植物内生细菌的群落结构与生长环境密切相关。
Cymbidium faberi (Cymbidium faberi), one of the representatives of the genus Cymbidium, is of high ornamental value and economic value. Studying its endophytic bacteria can not only enrich endophytic bacteria resources, but also explore the relationship between orchids and microorganisms Provide the basis for the interaction between the data. In this study, the isolation and culture methods and 16S rRNA gene sequence determination of Cymbidium wild Cymbidium, cultured in greenhouse one year after the endoscopic Cymbidium root endophytic bacteria genetic diversity were studied. The results showed that 97 strains of bacteria isolated from wild Cymbidium belonged to 13 genera of α-proteobacteria, β-proteobacteria, γ-proteobacteria and Firmicutes in Proteobacteria. The dominant groups were γ-Proteobacteria (86.60%) and Lelliottia (26.80%) were the dominant species. The 52 strains of bacteria isolated from Cymbidium sinense in greenhouse belong to 9 genera of Proteobacteria, β-proteobacteria, γ-proteobacteria and actinomycetes in Proteobacteria. The dominant groups are β - Proteobacteria (48.08%), dominant bacteria is Herbaspirillum (34.62%), of which strain eh R17 is a potential new species. These results indicated that the diversity of endophytic bacteria in cultivated roots of Cymbidium hybridium in Tianmu Mountain was more abundant than that in the greenhouse one year after cultivation. It also indicated that the community structure of plant endophytic bacteria was closely related to the growth environment.