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目的 :探讨转染人乳头瘤病毒 (HPV)的小鼠宫颈癌U1 4移植同系小鼠后生长特性和转移规律。方法 :用电穿孔法及阳离子脂质体将HPV 1 6型的早期基因E6 ,E7分别转染小鼠宫颈癌U1 4,筛选、鉴定表达E6 ,E7的转化细胞即E6 +U1 4,E7+U1 4,然后分别经皮下和腹腔移植于同系小鼠 ,观察其生长特性和转移规律。结果 :皮下移植野生型U1 4,E6 +U1 4及E7+U1 4后 ,出瘤时间分别为 5～ 7d ,1 1～ 1 4d及 8～ 1 0d (P <0 .0 5 ) ;平均生存期分别为 2 9d ,43 .3d及 3 5d ;淋巴转移率分别为 90 % ,3 0 %及 40 % ;肺转移率分别为 6 0 % ,1 0 %及 2 0 %。腹腔内移植后 ,荷瘤小鼠平均寿命分别为 1 4.2d ,2 0 .6d及 1 8.3d ;未发现淋巴和肺转移。结论 :转染HPV的小鼠宫颈癌U1 4移植同系小鼠后 ,显示出与移植野生型不同的生长特性和转移规律。该模型为研究以HPVE6 ,E7为靶的免疫或其他方法治疗宫颈癌提供了有价值的动物模型。
Objective: To investigate the growth characteristics and metastasis of mouse cervical U1 4 transplanted with human papillomavirus (HPV) in mice. Methods: The HPV16 E6 and E7 genes were transfected into U14 mouse cervical cancer cells by electroporation and cationic liposome respectively. The transformed cells expressing E6 and E7 were identified as E6 + U14 and E7 + U1 4, and then were subcutaneously and intraperitoneally transplanted in homologous mice, observe the growth characteristics and metastasis. RESULTS: After subcutaneous transplantation of wild-type U1 4, E6 + U1 4 and E7 + U1 4, the time to tumorigenesis was 5 to 7 days, 11 to 14 days and 8 to 10 days respectively (P <0.05); mean survival The rates of lymphatic metastasis were 90%, 30% and 40% respectively, and the rates of lung metastases were 60%, 10% and 20% respectively. After intraperitoneal transplantation, the average life span of tumor-bearing mice was 12.2d, 20.6d and 18.3d, respectively; no lymph and lung metastases were found. CONCLUSION: Mouse cervical cancer U14 transfected with HPV shows different growth characteristics and metastasis patterns compared with the wild type after transplantation. This model provides a valuable animal model for studying the treatment of cervical cancer with HPVE6, E7 target immune or other methods.