论文部分内容阅读
通过农杆菌介导法以花生下胚轴为外植体转化花生品种四粒红。经过共培养、抑菌和筛选培养,在含有0.8 mg/L Basta的培养基获得67株抗性再生植株。经PCR检测,获得5株阳性植株,bar试纸条检测至少有2株转基因植株为阳性。该体系操作简易、高效,可作为利用基因工程对花生进行研究和遗传改良的一种技术手段。
Agrobacterium-mediated transformation of peanut hypocotyls explants for the transformation of peanut varieties four red. After co-cultivation, antibacterial and screening culture, 67 resistant regenerated plants were obtained in medium containing 0.8 mg / L Basta. Five positive plants were obtained by PCR assay. At least two transgenic plants tested positive by bar test strip. The system is simple and efficient and can be used as a technical means to study and genetic improve peanut using genetic engineering.