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荧光分析法在测量混合物体系时,有些组分的最大激发峰相距较近,彼此之间会因存在干扰而得不着准确的荧光光谱。以中药苦参沸水浸取液为例,介绍了混合物体系中消除干扰的一种新方法。首先通过扫描苦参水浸液的荧光光谱,发现其中一种组分B对另一种组分A有明显干扰;然后通过几何作图法在组分B激发光谱中找到具有与组分A最大激发波长处同样荧光强度(也就是与在该点等高的位置处)的波长,以该波长作为激发波长扫描得到组分B在该波长处激发而得到的发射光谱;最后以受干扰的组分A的发射光谱减去该发射光谱即得到纯组分A的发射光谱。该方法使得在无法得到组分A和B的纯品时,对二者进行单独的荧光分析成为了可能。
Fluorescence analysis When measuring the mixture system, some of the components of the maximum excitation peak close to each other because of the interference can not get accurate fluorescence spectrum. Taking Chinese Sophora flavescens boiling water as an example, a new method to eliminate interference in mixture system was introduced. First, by scanning the fluorescence spectra of aqueous extract of Sophora flavescens, we found that one of the components B had a significant interference with the other component A. Then, by means of geometric mapping, we found in the excitation spectrum of component B The wavelength of the same fluorescence intensity at the excitation wavelength (that is, the position at the same height as that of the fluorescence intensity) is used as the excitation wavelength to obtain the emission spectrum of the component B excited at the wavelength; finally, Sub-A emission spectrum minus the emission spectrum obtained pure component A emission spectrum. This method makes it possible to perform a separate fluorescence analysis of both components when pure fractions of components A and B can not be obtained.