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以双系列线性回归法在显色剂准确浓度未知的情况下研究了Ｐｒ￣（３＋）与三溴偶氮肿（ＴＢＡ）的络合反应。在１２ｍｏｌ／Ｌ的ＨＣＩ介质中，侧得Ｐｒ￣（３＋）与ＴＢＡ形成１：３的络合物，条件稳定常数ｌｇβ＿３＝１５４７，摩尔吸光系数．
The complexation of Pr¯ (3+) with tribromoazoamylase (TBA) was studied by a series of linear regression methods at unknown chromogenic reagent concentrations. In the 12 mol / L HCI medium, Pr¯ (3+) forms a 1: 3 complex with TBA, and the conditional stability constant lgβ_3 = 1547, the molar extinction coefficient.