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目的 建立高效液相色谱法测定家犬血浆中灯盏乙素的浓度 ,研究灯盏乙素在家犬体内的药动学。方法 用高效液相色谱法测定 6只家犬静脉注射灯盏乙素后不同时间血浆中灯盏乙素的浓度 ,绘制药 -时曲线 ,计算药动学参数。结果 灯盏乙素的药 -时曲线符合三室模型 ,其T1 2 pi、T1 2 α和T1 2 β分别为 1.0 5± 0 .80min ,6 .99± 2 .76min和 5 1.6 1± 2 8.78min ;Vc为 880 .1± 5 0 8.3mL ;CL为 189.6± 5 3.8mL·min- 1 ;AUC0 90 和AUC0 ∞ 分别为 5 74 .4 3± 133.95 μg·min·mL- 1 和 5 99.34± 132 .0 0 μg·min·mL- 1 。结论 静脉注射给药后 ,血浆中灯盏乙素浓度迅速下降。灯盏乙素在家犬体内消除较快 ,提示临床给药方法或给药间隔时间的确定、制剂开发的剂型选择都应该考虑其T1 2 。
Objective To establish a HPLC method for the determination of scutellarin in dog plasma and study the pharmacokinetics of scutelin in dogs. Methods The plasma concentration of scutellarin in 6 dogs at different times after intravenous injection of scutellarin was determined by high performance liquid chromatography. The pharmacokinetic parameters were calculated by plotting the drug-time curve. Results The drug-time curve of scutellarin was in accordance with the three-compartment model. The T1 2 pi, T1 2 α and T1 2 β were 1.0 5 ± 0.80min, 6.99 ± 2.76min and 5 1.6 1 ± 2 8.78min, respectively. Vc was 880.1 ± 5 0 8.3 mL; CL was 189.6 ± 5 3.8 mL · min-1; AUC0 90 and AUC0∞ were 5 74.33 ± 133.95 μg · min · mL -1 and 5 99.34 ± 132, respectively. 0 0 μg · min · mL-1. Conclusion After intravenous injection, the concentration of scutellarin rapidly decreases in plasma. Scutellarin in dogs faster elimination of body, suggesting that clinical methods of administration or interval to determine the formulation developed formulations should consider the T1 2.