UGPase(UDP-glucose pyrophosphorylase,尿苷二磷酸葡萄糖焦磷酸化酶)是糖代谢过程中的关键酶,对蔗糖积累和细胞壁形成有重要影响.将处于生长期的甘蔗叶片经蔗糖、光照、冷诱导等条件处理后,测定UGPase酶活性,并利用real-time PCR技术同步测定Ugp的相对表达量.研究结果表明:环境中的蔗糖、低温(4℃)、光照对UGPase基因的表达调控呈上调,对UGPase酶活性有提高的作用. UGPase (UDP-glucose pyrophosphorylase) is a key enzyme in the process of sugar metabolism, which has an important effect on sucrose accumulation and cell wall formation. The sugarcane leaves in the growth phase were induced by sucrose, light and cold UGPase activity was assayed and the relative expression level of Ugp was measured simultaneously by real-time PCR.The results showed that the regulation of UGPase gene expression was upregulated in the environment of sucrose, low temperature (4 ℃) UGPase activity on the role of increased.