论文部分内容阅读
目的:调查我国四川地区妇女宫颈癌组织中HPV33、52和58型的感染率及E6基因结构特点,探讨其与宫颈癌发生的关系。方法:采用PCR技术,对四川地区2004年60例宫颈癌患者癌组织DNA进行HPV检测。结果:获得HPV52和HPV58型各4例,检出率为6．7％;HPV33型1例,检出率为1．7％。对所获得的各例 HPV33、52和58 E6基因测序分析。结论:与Genbank标准株相比,四川地区发现的HPV33和HPV52 E6基因有多处突变,与日本发现的HPV33,52突变株相近。
Objective: To investigate the infection rate of HPV33, 52 and 58 and the structural features of E6 in women with cervical cancer in Sichuan in China and to explore their relationship with the occurrence of cervical cancer. Methods: PCR was used to detect HPV in 60 cases of cervical cancer in Sichuan Province in 2004. Results: Four cases of HPV52 and HPV58 were obtained, the detection rate was 6.7%. One case of HPV33 was detected with a detection rate of 1.7%. The obtained HPV33, 52 and 58 E6 gene sequences were analyzed. Conclusion: Compared with GenBank, a number of mutations of HPV33 and HPV52 E6 genes were found in Sichuan Province, similar to the HPV33 and 52 mutations found in Japan.