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目的 探讨甘露醇对大鼠局灶性脑缺血再灌注损伤脑组织NO及神经细胞凋亡的影响。方法 SD大鼠 4 8只 ,随机等分成 4组 ,每组 12只。A组 :假手术组 ;B组 :模型组 ,即局灶性脑缺血再灌注组 ;C组 :小剂量甘露醇组 ;D组 :大剂量甘露醇组。采用硝酸还原酶法及TUNEL法分别检测各组大鼠脑组织NO含量及神经细胞凋亡数目。结果 B组脑组织NO含量及神经细胞凋亡数目均明显多于A组 (P <0 .0 1) ;C组、D组脑组织NO含量及神经细胞凋亡数目均较B组减少 (P <0 .0 5 ) ;C组与D组脑组织NO含量及神经细胞凋亡数目比较 ,无显著差异 (P >0 .0 5 )。结论 甘露醇的脑保护作用不完全与脱水降颅压有关 ,还可能与下调缺血再灌注后脑组织NO含量、抑制神经细胞凋亡有关。
Objective To investigate the effect of mannitol on the apoptosis of NO and nerve cells in rats with focal cerebral ischemia-reperfusion injury. Methods Forty-eight SD rats were randomly divided into four groups (n = 12). Group A: sham operation group; Group B: model group, focal cerebral ischemia-reperfusion group; Group C: low dose mannitol group; Group D: high dose mannitol group. Nitric acid reductase method and TUNEL method were used to detect the content of NO in brain tissue and the number of neuronal apoptosis in each group. Results Compared with group A, the content of NO and the number of neuron apoptosis in group B were significantly higher than those in group A (P <0.01). The content of NO and the number of neuronal apoptosis in group C and group D were lower than those in group B <0.05). There was no significant difference in the content of NO and the number of neuronal apoptosis between groups C and D (P> 0.05). Conclusion The protective effect of mannitol on brain is not completely related to dehydration and intracranial pressure. It may be related to the decrease of NO content in brain tissue and the inhibition of neuronal apoptosis after ischemia / reperfusion.