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肝星状细胞是肝脏中重要的间质细胞,是肝细胞外基质的主要来源.表皮形态发生素(epimorphin、EPM、syntaxin2)在肝脏发育、再生及癌变过程中发挥了重要的作用,目前其表达变化的调控机制及对肝星状细胞的作用还未有报道.通过对肝组织标本进行检测,发现肝纤维化过程中肝星状细胞表达EPM上调.从表观遗传学的角度对EPM表达变化调控机制进行研究,发现DNA去甲基化促进了EPM的表达.为了研究EPM对肝星状细胞的可能的调节作用,将EPM表达质粒转染肝星状细胞,之后检测了EPM对肝星状细胞增殖及迁移能力的变化.结果证明EPM能够促进肝星状细胞的增殖与迁移.本研究发现,激活的肝星状细胞高表达EPM可能是由于DNA去甲基化引起的,同时,高表达的EPM能够促进肝星状细胞的增殖与迁移,进而促进肝纤维化进展.
Hepatic stellate cells are the important interstitial cells in the liver and are the main source of extrahepatic extracellular matrix. Epimorphin (EPM, syntaxin2) plays an important role in liver development, regeneration and carcinogenesis. At present, Up-regulation of expression and its effect on hepatic stellate cells have not been reported yet.Expression of EPM in hepatic stellate cells during hepatic fibrosis was detected by detecting hepatic tissue samples.Expression of EPM from the epigenetic point of view We found that demethylation of DNA promoted the expression of EPM.In order to study the possible regulation of EPM on hepatic stellate cells, EPM expression plasmids were transfected into hepatic stellate cells, and then the effects of EPM on hepatic stellate cells EPM can promote the proliferation and migration of hepatic stellate cells.In this study, it was found that high expression of EPM in activated hepatic stellate cells may be due to DNA demethylation, meanwhile, high The expressed EPM can promote the proliferation and migration of hepatic stellate cells and further promote the progress of hepatic fibrosis.