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目的评价贝那普利对兔急性心肌梗死(AMI)再灌注后内皮素-1(endothelin-1,ET-1)的影响及对无复流的防治作用。方法新西兰大白兔24只随机分成对照组、贝那普利组[3 mg/(kg.d)]和假手术组,每组8只。将冠状动脉结扎60 min、松解120 min,制备AMI再灌注模型。梗死前、后和再灌注后,用导管法进行血液动力学测定。采用放射免疫测定法检测血浆及心肌组织中ET-1的水平,最终进行病理学分析。结果与对照组相比,贝那普利组能明显降低兔AMI再灌注后血浆ET-1的水平(P<0.01);而心肌组织中ET-1的含量仅复流区显著降低(P<0.01)。与对照组相比,贝那普利可促进AMI后心功能的恢复,减少无复流的面积[对照组为75.3%结扎区(LA),贝那普利组为35.9%LA,P<0.01]。结论贝那普利能有效降低血浆及心肌组织中ET-1的水平,具有内皮保护作用;能有效地防治心肌梗死再灌注后无复流现象的发生。
Objective To evaluate the effects of benazepril on endothelin-1 (ET-1) and its prevention and treatment after acute myocardial infarction (AMI) reperfusion in rabbits. Methods Twenty-four New Zealand white rabbits were randomly divided into control group, benazepril group [3 mg / (kg · d)] and sham operation group, with 8 rats in each group. The coronary artery ligation 60 min, release 120 min, AMI reperfusion model. Before infarction, after and after reperfusion, the catheter method was used for hemodynamic determination. The level of ET-1 in plasma and myocardium was detected by radioimmunoassay and pathological analysis was finally performed. Results Compared with the control group, the benazepril group could significantly decrease the level of plasma ET-1 after AMI reperfusion (P <0.01), while the content of ET-1 in myocardial tissue only decreased significantly (P < 0.01). Compared with the control group, benazepril promoted the recovery of cardiac function after AMI and reduced the area without reflow (75.3% in the control group, 35.9% LA in the benazepril group, P <0.01) ]. Conclusions Benalipril can effectively decrease the level of ET-1 in plasma and myocardium, and has the protective effect on endothelial cells. It can effectively prevent and treat the occurrence of no-reflow after myocardial infarction reperfusion.