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我们以实验性慢性胃溃疡大鼠为动物模型,用酶组织化学和免疫组织化学方法,观察了胃溃疡自愈过程中睾丸间质细胞内合成睾酮的关键酶3β-羟基类固醇脱氢酶(3βHSD)及睾酮的改变。实验用雄性Wistar大鼠100只,分为正常对照组、盐水对照组和胃溃疡组。各组分别于术后4、6、10、14、21和28d测溃疡面积。取右侧睾丸恒冷箱切片,用四唑盐法显示间质细胞3βHSD,用半定量积分法评价其活性。左侧睾丸经4%多聚甲醛
Using an experimental chronic gastric ulcer rat as an animal model, the enzyme 3β-hydroxysteroid dehydrogenase (3βHSD), a key enzyme in the synthesis of testosterone in testicular stromal cells, was observed during enzymatic histochemistry and immunohistochemistry. ) and changes in testosterone. One hundred male Wistar rats were used in the experiment and divided into normal control group, saline control group, and gastric ulcer group. The ulcer area was measured at 4, 6, 10, 14, 21, and 28 days after operation in each group. Take the right testis cryostat section, use tetrazolium salt method to show the interstitial cells 3βHSD, semi-quantitative integration method to evaluate its activity. Left testis with 4% paraformaldehyde