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用 60个随机引物对鳜SinipercachuatsiBasilewsky野生群体和养殖群体进行随机扩增多态DNA(RAPD) 分析, 经过引物筛选, 分别用 55个和 54个引物对野生鳜和养殖鳜进行群体内分析。结果表明, 野生群体多态位百分率为 85 75%, 群体内遗传相似率和遗传距离分别为 0 8547和 0 1453; 养殖群体多态位百分率为 16 39%, 遗传相似率和遗传距离分别为 0 9527和 0 0473; 两群体间遗传相似率和遗传距离分别为 0 6905和 0 3095; 鳜野生群体具有较高的遗传多样性, 养殖群体的遗传多样性却显著降低, 野生群体与养殖群体间有明显的遗传差异, 说明人工繁育对鳜基因组造成了显著的影响。
Random amplified polymorphic DNA (RAPD) analysis was performed on wild and cultured populations of ini SinipercachuatsiBasilewsky by using 60 random primers. After primer selection, 55 and 54 primers were used to analyze the wild 鳜 and cultured 鳜 respectively. The results showed that the percentage of polymorphic loci in wild populations was 85 75%, the genetic similarity and genetic distance within populations were 0 8547 and 0 1453, respectively. The percentage of genetic diversity in breeding population was 16 39%, and the genetic similarity and genetic distance were 0 9527 and 0 0473 respectively. The genetic similarity and genetic distance between the two groups were 0 6905 and 0 3095, respectively. The wild populations had high genetic diversity, while the genetic diversity of farmed populations was significantly reduced. Significant genetic differences, indicating that artificial reproduction of the 鳜 genome caused a significant impact.