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本文利用 rep- PCR指纹分析方法研究了我国北方部分稻区稻瘟病菌的单元型 (haplotype)和谱系 (lineage)。根据单元型和谱系组成以及优势单元型和优势谱系的变化 ,分析了稻瘟病菌的群体结构和遗传变异性 ,证实了稻瘟病菌在 DNA水平上是高度异质和极易变异的。对病原菌群体的时空变化分析发现 ,稻瘟病菌群体的单元型和谱系因年度和地区而不同。不同年度、不同地区的稻瘟病菌亚群体间几乎无共同的单元型和谱系。对 138个供试菌株的致病型分析发现 ,单元型和谱系与致病型之间不存在规律性的对应关系 ,这说明用 rep- PCR方法揭示的稻瘟病菌的遗传变异很可能与致病性变异不存在必然的联系
In this paper, the haplotypes and lineages of Magnaporthe grisea in northern part of China were studied using rep-PCR fingerprinting. The population structure and genetic variability of Magnaporthe grisea were analyzed based on the changes of haplotypes and pedigree composition, dominant haplotypes and dominant lineages, and confirmed that M. grisea was highly heterogeneous and highly variable at the DNA level. Analysis of the spatiotemporal variation of pathogen populations found that the haplotype and lineage of the M. grisea population varied with the year and the region. In different years, there are almost no common haplotypes and pedigrees in the rice blast populations in different regions. The pathogenicity analysis of 138 tested strains showed no regular correspondence between haplotype and pedigree and pathogenicity, which indicated that the genetic variation of M. grisea revealed by rep- There is no necessary relationship between pathological variation