论文部分内容阅读
目的研究脑梗死患者外周血磷酸二酯酶(PDE)活性的变化及意义。方法应用高效液相色谱法(HPLC)检测298例脑梗死患者和181例对照组外周血白细胞的PDE活性,同时检测他们的血生化指标,收集临床资料,进行统计分析。结果脑梗死组外周血PDE活性为(13.78±3.12),对照组为(7.36±2.34),差异有统计学意义(P<0.01),但外周血PDE活性与病情的严重程度无关。Logistic回归分析表明,外周血PDE活性水平与脑梗死有关(P<0.01)。结论脑梗死患者PDE活性增高,HPLC能简单易行、精密可靠地测定外周血白细胞的PDE活性,PDE在脑梗死的发病中可能起重要作用。
Objective To study the changes and significance of phosphodiesterase (PDE) activity in peripheral blood of patients with cerebral infarction. Methods The peripheral blood leukocytes in 298 patients with cerebral infarction and 181 controls were assayed for PDE activity by high performance liquid chromatography (HPLC). Meanwhile, their blood biochemical parameters were measured and clinical data were collected for statistical analysis. Results The PDE activity in the peripheral blood was (13.78 ± 3.12) in the infarction group and (7.36 ± 2.34) in the control group (P <0.01). However, the PDE activity in peripheral blood was not related to the severity of the disease. Logistic regression analysis showed that PDE activity in peripheral blood was related to cerebral infarction (P <0.01). Conclusion PDE activity in patients with cerebral infarction increased, HPLC can be simple and easy, accurate and reliable determination of PDE activity in peripheral leukocytes, PDE may play an important role in the pathogenesis of cerebral infarction.