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活性诱导物几丁质低聚糖是否参与了植物对聚合物的反应，或是否在真菌方面有重要性尚不清楚．在本试验中，作者调查了活性诱导物几丁质低聚糖Ｎ－乙酰－Ｄ－氨基葡糖（ＧｌｃＮＡｃ）４－６在损伤小麦叶片中保护性本质化诱导的重要性．其目的是探讨用几丁质处理后，在植物体内是否能检测出活性诱导的低聚糖，以及所检测的水平是否能说明由几丁质诱导的木质化程度．用几丁质或几丁质四聚物处理的小麦叶片，其几丁质低聚糖含量通过组织提取物的ＨＰＬＣ分析进行检测。几丁质处理叶片中低聚物的分布表明，可能是由于几丁质酶及其它水解酶的作用，聚合体水解产生了单体和至少为六聚体的低聚物．当施用到叶片时，随着非诱导的主要产物单体及二聚体的产生，四聚体也被水解．当施用足以诱导木质化的（ＧｌｃＮＡｃ）浓度时，１２ｈ后组织的（ＧｌｃＮＡｃ）４含量比在叶片中经几丁质处理后产生的活性诱导物低聚糖（ＧｌｃＮＡｃ）４－６的混合含量要高得多．有意义的是，当施用低浓度不诱导木质化的（ＧｌｃＮＡｃ）４时，所测定的（ＧｌｃＮＡｃ）４含量仍比在叶片中用诱导木质化的几丁质浓度处理产生的活性诱导低聚糖（ＧｌｃＮＡｃ）４－６的含量高得多．因此，试验的证据并不支持以往的活性诱导物（Ｇｌｃ?
It is unclear whether the active inducer chitin oligosaccharide is involved in the plant response to the polymer, or whether it is fungally important. In this experiment, the authors investigated the importance of protective inducible induction of the active inducer chitooligosaccharides N-acetyl-D-glucosamine (GlcNAc) 4-6 in injured wheat leaves. The aim was to investigate whether activity-induced oligosaccharides can be detected in plants after treatment with chitin and whether the level of lignin detected by chitin can be used to explain the degree of lignification induced by chitin. The chitin oligosaccharide content of wheat leaves treated with chitin or chitin tetramer was determined by HPLC analysis of tissue extracts. The distribution of oligomers in the chitin treated leaves suggests that the hydrolysis of the polymer produces monomers and oligomers of at least hexamers, possibly due to the action of chitinase and other hydrolases. Tetramers are also hydrolyzed as non-induced production of major product monomers and dimers when applied to the leaves. The (GlcNAc) 4 content of the tissues after 12 h was greater than that of the active inducer oligosaccharides (GlcNAc) 4-6 produced after chitin treatment in the leaves when a concentration of (GlcNAc) sufficient to induce lignification was administered Much higher. Significantly, the (GlcNAc) 4 content determined (GlcNAc) 4 was still lower than that of the active-induced oligosaccharides produced by treatment with the lignin-inducing chitin concentration in the leaves when low concentrations of lignified (GlcNAc) 4 were not applied (GlcNAc) 4-6 content is much higher. Therefore, the experimental evidence does not support the past activity of the inducer (Glc?