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目的探讨田黄片(THP)对血管内皮损伤后内膜增生兔模型的影响。方法利用球囊损伤术结合高脂喂养建立血管内皮损伤后内膜增生模型,28只新西兰白兔随机分为对照组、模型组、THP组(0.3 g·kg~(-1))和阿托伐他汀组(0.5 mg·kg~(-1))。通过血管造影分析计算病变点直径相对血管腔参考直径的比例(%MLD)、病变点面积相对血管腔参考面积的比例(%MLA);取腹主动脉进行病理检查,并测量血管内膜厚度及内膜中膜厚度比值(内膜厚度/中膜厚度);检测血清中过氧化氢酶(CAT)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)、一氧化氮(NO)、内皮素-1(ET-1)的水平。结果与对照组相比,模型组的%MLD、%MLA、内膜厚度、内膜中膜厚度比值明显升高(P<0.05),内膜增生明显;血清中的SOD、CAT、GHS-Px、NO水平降低(P<0.05),MDA、ET-1水平升高(P<0.05),血管的抗氧化能力降低、内皮功能受损。与模型组比较,THP组可以显著降低%MLD、%MLA、内膜厚度、内膜中膜厚度比值(P<0.05),改善血管的内膜增生状况;病理检查发现,THP组内膜增厚情况较模型组明显减轻,内膜平整,炎症细胞侵入及平滑肌细胞迁移明显减少;THP组血清SOD、CAT、GSH-Px、NO含量明显升高,MDA、ET-1含量明显降低,差异均有统计学意义(P<0.05)。结论 THP可以抑制血管内皮损伤后内膜的进一步增生,其作用机制可能与改善血管的抗氧化能力,保护血管内皮功能有关。
Objective To investigate the effect of Tianhuang Tablets (THP) on intima hyperplasia rabbit model after vascular endothelial injury. Methods The neointimal hyperplasia model was established by balloon injury combined with high fat diet. 28 New Zealand white rabbits were randomly divided into control group, model group, THP group (0.3 g · kg -1) In the statin group (0.5 mg · kg -1). The ratio of lesion point diameter to reference vessel diameter (% MLD) and the ratio of lesion area to reference vessel area (% MLA) were calculated by angiographic analysis. The abdominal aorta was taken for pathological examination and the intima-media thickness Intima-media thickness ratio (intima-media thickness) was measured. Serum levels of catalase (CAT), malondialdehyde (MDA), glutathione peroxidase (GSH-Px), superoxide (SOD), nitric oxide (NO), endothelin-1 (ET-1) levels. Results Compared with the control group, the% MLD,% MLA, intima-media thickness and intima-media thickness ratio in the model group were significantly increased (P <0.05), and intimal hyperplasia was obvious. Serum SOD, CAT, GHS-Px , NO level decreased (P <0.05), MDA, ET-1 level increased (P <0.05), blood vessel antioxidant capacity decreased, endothelial dysfunction. Compared with the model group, THP group could significantly reduce% MLD,% MLA, intima-media thickness and intima-media thickness ratio (P <0.05), and improve the intimal hyperplasia of blood vessels. The content of SOD, CAT, GSH-Px and NO in THP group were significantly increased, while the content of MDA and ET-1 in THP group was significantly lower than that in model group Statistical significance (P <0.05). Conclusions THP can inhibit the intimal hyperplasia after vascular endothelial injury, and its mechanism may be related to the improvement of anti-oxidative ability and protection of vascular endothelial function.