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采用PCR SSO和PCR RFLP技术对 6 0例经临床及肾穿刺证实的IgA肾炎 (IgAN )患者和 80例正常人的TAP、LMP2和DM等位基因频率进行了检测。结果显示在患者和正常人中各等位基因频率均无显著性差异 ,表明IgAN与抗原处理相关基因无关联。同时分析了经典Ⅱ类基因 (DR/DQ/DP )和抗原处理相关基因 (TAP/LMP2 /DM )之间可能存在的连锁不平衡 ,获得了扩展的HLAⅡ类单倍型 ,为HLAⅡ类基因与某些自身免疫病的关联性研究提供了有价值的数据
The frequency of TAP, LMP2 and DM alleles in 60 patients with IgA nephritis (IgAN) confirmed by clinical and renal puncture and 80 normal controls were detected by PCR SSO and PCR RFLP. The results showed no significant difference in allele frequencies between patients and normal subjects, indicating that IgAN is not associated with antigen-related genes. At the same time, we analyzed possible linkage disequilibrium between classical class Ⅱ gene (DR / DQ / DP) and antigen-related gene (TAP / LMP2 / DM) and obtained extended HLA Ⅱ haplotype. Relevant studies of some autoimmune diseases provide valuable data