论文部分内容阅读
成年SD大鼠于第九胸髓(T9)处横断损伤,一周后将大鼠胚胎脑干中缝组织细胞悬液植入T9以下的脊髓内,动物存活1~12月,分期处死取材,作5-羟色胺(5-HT)免疫组化的光镜和电镜观察。结果:脊髓损伤后在损伤部位尾侧的脊髓内5-HT阳性标记的纤维末梢消失,移植后于植入部位见许多5-HT阳性标记细胞,其突起在脊髓灰质内长距离延伸,末梢的分布与正常动物相似,并在免疫电镜下见到5-HT能神经末梢的突触形成。
Adult Sprague-Dawley rats were transected at the ninth thoracic bone (T9). One week later, the interstitial cells of the seminal vesicles in the rat embryos were implanted into the spinal cord below T9. The animals survived from January to December and were sacrificed by staging. Immunohistochemistry of Serotonin (5-HT) by Light and Electron Microscopy. Results: The 5-HT-labeled fibers in the caudal spinal cord disappeared after spinal cord injury. Many 5-HT positive labeled cells were observed at the implantation site after implantation. The protrusions extended long-distance within the gray matter of the spinal cord. Distribution similar to normal animals, and seen in the immunoelectron microscopy 5-HT nerve endings synapse formation.