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目的:测定天山雪莲不同部位的总黄酮和芦丁含量,以及高效液相指纹图谱分析,为雪莲叶进一步开发利用奠定基础。方法:用UV法测定总黄酮的含量;用中药色谱指纹图谱相似度评价系统,对不同部位的HPLC色谱图进行相似度计算。结果:叶的总黄酮和芦丁的含量均较高,与全草的相似度为0.812。
Objective: To determine the content of total flavonoids and rutin in different parts of Saussurea involucrata, and to analyze the HPLC fingerprints, so as to lay a foundation for further development and utilization of Saussurea. Methods: The content of total flavonoids was determined by UV method. The similarity degree was calculated by using similarity evaluation system of chromatographic fingerprints of traditional Chinese medicines. Results: The contents of total flavonoids and rutin in the leaves were high, and the similarity to the whole grass was 0.812.