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目的:探讨FN及整合素β1在病毒性心肌炎心肌纤维化中的表达及清心Ⅱ号的影响。方法:建立病毒性心肌炎慢性期心肌纤维化模型后小鼠随机分为模型组、卡托普利治疗组、清心Ⅱ号高、中、低灌胃治疗组。疗程结束后分别采集心脏,应用免疫组化技术检测FN、免疫荧光及激光共聚焦法检测基质连接蛋白整合素β1。结果:造模后FN及整合素β1均明显增高,应用RT-PCR在心肌中可检测出病毒,清心Ⅱ号干预后FN及整合素β1减低,各治疗组病毒减少。结论:病毒、FN及其整合素β1在心肌纤维化形成中具有重要作用,清心Ⅱ号对其干预作用说明在抗病毒、抗心肌纤维化中具有一定作用。
Objective: To investigate the expression of FN and integrin β1 in myocardial fibrosis of viral myocarditis and the effect of Qingxin II. METHODS: After establishment of a chronic myocardial fibrosis model of viral myocarditis, mice were randomly divided into a model group, a captopril treatment group, and a Qingxin II high, middle, and low gavage treatment group. At the end of the treatment period, hearts were collected and FN, immunofluorescence and confocal laser scanning confocal microscopy were used to detect the integrin β1. RESULTS: FN and integrin β1 were significantly increased after modeling. Viruses were detected in the myocardium using RT-PCR. FN and integrin β1 were decreased after Qingxin II intervention. The virus in each treatment group was decreased. Conclusion: The virus, FN and its integrin β1 play an important role in the formation of myocardial fibrosis. The intervention of Qingxin II shows that it plays a role in anti-virus and anti-myocardial fibrosis.