目的建立紫外分光光度法测定枸杞多酚提取物中的总多酚。方法采用紫外分光光度法测定枸杞多酚提取物中总多酚,用水稀释样品,加入显色剂Filin-Ciocalteu试剂,在760 nm处测定吸光度,用同样方法测定并绘制标准曲线。结果紫外分光光度法测定枸杞多酚提取物中总多酚的量分别为24.03%、26.38%、25.45%、28.27%、29.08%,线性范围1～8μg/mL,R2=0.999 5。平均回收率为103.5%,RSD<5%。结论该方法操作简单,准确可靠,适于枸杞多酚提取物中总多酚的测定。 Objective To establish a UV spectrophotometric method for the determination of total polyphenols in Lycium barbarum polyphenol extract. Methods The total polyphenols in Lycium barbarum L. extract were determined by UV spectrophotometry. The samples were diluted with water and the reagent Filin-Ciocalteu was added to determine the absorbance at 760 nm. The standard curve was determined and plotted in the same way. Results The total polyphenols of Lycium barbarum polysaccharide extracts were 24.03%, 26.38%, 25.45%, 28.27%, 29.08%, respectively. The linear range was 1 ~ 8μg / mL and R2 was 0.999 5 by UV spectrophotometry. The average recovery was 103.5% with RSD <5%. Conclusion The method is simple, accurate and reliable, suitable for the determination of total polyphenols in the extract of Lycium barbarum L.