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为了获取植物根际高效溶磷菌,试验对松嫩草地乡土植物羊草(Leymus chinensis)和星星草(Puccinellia tenuiflora)进行了根际土壤取样,分离纯化,测定了羊草和星星草根际溶磷菌分解无机磷的能力,并对溶磷菌的生长条件优化处理。结果表明:初步得到的溶磷菌株27株中,有效磷增加量超过150 mg/L的菌株有4株,聚类分析得到具备较强溶磷水平菌株1株。两种乡土植物根际菌株的溶磷能力与p H值具有线性关系(P<0.05),并且星星草根际菌的溶磷量与p H值之间的线性关系较强(R2=0.425)。供试菌株19生长的适宜碳源和氮源分别为麦芽糖和尿素,优化培养基是麦芽糖、尿素、Na Cl用量分别为5,0.05,5 g/L,p H值为5,在30℃下培养48 h菌落总数可达6.03×109cfu/m L。
In order to obtain high efficient phosphate-solubilizing bacteria in plant rhizosphere, rhizosphere soil was sampled from Leymus chinensis and Puccinellia tenuiflora in Songnen grassland for isolation and purification. The rhizosphere phosphorus Bacteria decomposition of inorganic phosphorus ability, and the optimum conditions for the growth of phosphate-solubilizing bacteria. The results showed that there were 4 isolates in 27 strains of phosphorus-solubilizing bacteria that had an available phosphorus increase of more than 150 mg / L, and 1 isolates were obtained by cluster analysis. The phosphorus solubilities of rhizosphere strains of two native plants had a linear relationship with p H value (P <0.05), and there was a strong linear relationship between p amount and dissolved phosphorus (R2 = 0.425). The suitable carbon and nitrogen sources for the growth of the tested strain 19 were maltose and urea respectively. The optimal medium was maltose and urea. The dosage of NaCl was 5, 0.05, 5 g / L, p H value was 5, The total number of colonies cultured for 48 h reached 6.03 × 109 cfu / m L.