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目的:建立芪蛭固本通脉丸的质量标准。方法:采用薄层色谱法(TLC)对芪蛭固本通脉丸中的黄芪、党参、川芎、水蛭、金银花进行定性鉴别,采用HPLC法对芪蛭固本通脉丸中黄芪的有效成分黄芪甲苷以及金银花中的绿原酸分别进行含量测定。结果:薄层色谱专属性强,斑点清晰。黄芪甲苷在0.51～10.2μg(r=0.9990)范围内线性关系良好,平均回收率为95.55%(RSD=1.0%,n=9);绿原酸在0.077～2.322μg(r=0.9998)范围内线性关系良好,平均回收率为96.77%(RSD=1.3%,n=9)。结论:该方法简便快速,结果准确,可以有效控制芪蛭固本通脉丸质量。
Objective: To establish the quality standard of Qizhi Guben Tongmai pill. Methods: The contents of Radix astragali, Corydalis chinense, Rhizoma chuanxiong, Leech and Honeysuckle in Qizhi Guanyuantongmai pill were qualitatively identified by TLC. The contents of Radix Astragali Cytosine and chlorogenic acid in honeysuckle were determined. Results: Thin-layer chromatography is highly specific and spotted. Astragaloside had a good linear relationship in the range of 0.51-10.2μg (r = 0.9990) with an average recovery of 95.55% (RSD = 1.0%, n = 9); chlorogenic acid was in the range of 0.077-2.3328μg Within the linear relationship was good, the average recovery was 96.77% (RSD = 1.3%, n = 9). Conclusion: The method is simple and rapid with accurate results and can effectively control the quality of QLGGT.