目的:构建结核分枝杆菌培养滤液蛋白32(culture filtrate protein 32,CFP32)的重组质粒,在大肠杆菌中表达并纯化重组蛋白CFP32,探讨其在结核病血清学诊断中的应用价值。方法:PCR扩增CFP32基因片段,将其克隆到pET-21a表达载体。优化表达条件,Ni-NTA亲和层析方法纯化重组CFP32蛋白。建立检测CFP32抗体的酶联免疫吸附试验(ELISA)方法,并用于检
A clinical trial of radiotherapy with modified simultaneous integrated boost(SIB)technique against huge tumors was conducted.A 58-year-old male patient who had
AIM:To explore the potential of β-elemene as a radiosensitizer for gastric cancer cells and the underlying mechanisms.METHODS:SGC7901,MKN45,MKN28,N87,and AGS h