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目的:研究orexin-A对缺血再灌注大鼠脑损伤的保护作用。方法:取成年雄性大鼠6只,观察MCAO前和MCAO后2 h、24h的生理学参数,界定后续指标参考时间。另取20只大鼠随机分为MCAO组、vehicle组、orexin-A 50μg/kg组和orexin-A 100μg/kg组(n=5),于缺血再灌注24 h后评估大鼠神经功能学评分和脑梗死容积。再取60只大鼠同样分成4组,(各组n=15),每组在术前、手术后6 h、24 h(各时间点n=5)取脑组织匀浆离心,检测上清液中谷胱甘肽过氧化物酶(GSH-PX)和丙二醛(MDA)的含量。结果:(1)大鼠MCAO术前、术后2 h、24 h生理参数比较无统计学意义(P>0.05),提示脑保护参考指标在MCAO后24 h内不受影响。(2)与MCAO组、vehicle组相比,orexin-A 50和100μg/kg降低神经功能评分(P<0.05)且梗死容积缩小(P<0.05);术前、术后6 h和术后24 h,脑匀浆中GSH-PX活性升高,MDA含量降低(P<0.05)。结论:Orexin-A可能通过降低脑内自由基水平,控制脂质过氧化物酶从而对脑缺血再灌注损伤起保护作用。
Objective: To study the protective effect of orexin-A on brain injury induced by ischemia-reperfusion in rats. Methods: Six adult male rats were used to observe the physiological parameters of MCAO before and 2 h and 24 h after MCAO, and define the reference time of follow-up index. Another 20 rats were randomly divided into MCAO group, vehicle group, orexin-A 50μg / kg group and orexin-A 100μg / kg group (n = 5). Neurological function was assessed 24 hours after ischemia-reperfusion Scores and cerebral infarction volume. Sixty rats were equally divided into four groups (n = 15 in each group). Each group was centrifuged at 6 h and 24 h after surgery (n = 5 at each time point) The contents of glutathione peroxidase (GSH-PX) and malondialdehyde (MDA) in the fluid were measured. Results: (1) There was no significant difference in physiological parameters between preoperative and postoperative 2 h and 24 h after MCAO (P> 0.05), suggesting that the reference indexes of cerebral protection were not affected within 24 h after MCAO. (2) Compared with MCAO group and vehicle group, the neurological scores of orexin-A 50 and 100 μg / kg decreased (P <0.05) and infarct volume decreased (P <0.05) h, GSH-PX activity in brain homogenate increased and MDA content decreased (P <0.05). Conclusion: Orexin-A may protect cerebral ischemia-reperfusion injury by decreasing free radicals in brain and controlling lipid peroxidase.