论文部分内容阅读
目的探讨通过测定乳腺新鲜离体组织傅立叶变换红外光谱,区分乳腺癌和良性乳腺组织的可行性及机制。方法联合应用衰减全反射(ATR)探头与中红外光纤、傅立叶变换红外光谱仪,检测乳腺手术中切除的新鲜离体乳腺组织29例。比较良、恶性乳腺组织红外光谱各谱带的峰高和相对峰强。结果共得到红外光谱45张。乳腺癌组织与良性乳腺组织的新鲜离体标本红外光谱有明显差异。代表蛋白质结构的I1640／I1550和I1160／I1120、代表蛋白质与脂类相对含量的I1640／I1460和I1550／I1460、代表脂类结构的I1460／I1400以及代表核酸的I1310／I1240,乳腺癌组织与良性乳腺组织间差异均有统计学意义。结论傅立叶变换红外光谱技术可用于临床判别乳腺癌和良性乳腺组织,且具有快速、方便、无创伤的特点。
Objective To investigate the feasibility and mechanism of breast cancer and benign breast tissue by measuring Fourier transform infrared spectroscopy (FTIR) of fresh isolated breast tissue. Methods Combined with attenuated total reflection (ATR) probe and mid-infrared fiber, Fourier transform infrared spectroscopy, detection of fresh excised breast tissue in breast surgery in 29 cases. Relatively good, malignant breast tissue infrared spectra of the peak height and relative intensity. Results A total of 45 infrared spectra were obtained. Breast cancer and benign breast tissue fresh in vitro samples were significantly different infrared spectra. I1640 / I1550 and I1160 / I1120 representing the protein structure, I1640 / I1460 and I1550 / I1460 representing the protein and lipid relative content, I1460 / I1400 representing the lipid structure, and I1310 / I1240 representing the nucleic acid, breast cancer tissue and benign breast Differences between the two groups were statistically significant. Conclusion Fourier transform infrared spectroscopy can be used in clinical diagnosis of breast cancer and benign breast tissue, and has the characteristics of rapid, convenient and non-invasive.