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目的:探讨中国汉族人群载脂蛋白C3(-482C>T)多态性与非酒精性脂肪肝(nonalcoholic fatty liver disease,NAFLD)及其临床特征的关系。方法:采用聚合酶链式反应-限制性片段长度多态性,结合聚丙烯酰胺凝胶电泳技术分析300例NAFLD患者及300例健康对照者载脂蛋白C3(-482C>T)多态性。应用Logistic回归模型分析该位点多态性对NAFLD及其临床特征的影响。结果:载脂蛋白C3(-482C>T)基因型频率在NAFLD患者及健康对照人群间差异无统计学意义(P>0.05)。与野生纯合子CC相比,NAFLD患者T等位基因携带者更容易合并胰岛素抵抗、高血压、高甘油三酯血症或高密度脂蛋白胆固醇降低。结论:载脂蛋白C3(-482C>T)多态性与中国汉族人NAFLD易感性不相关,但T等位基因携带者增加NAFLD患者合并代谢异常的风险。
Objective: To investigate the association between apolipoprotein C3 (-482C> T) polymorphism and nonalcoholic fatty liver disease (NAFLD) in Chinese Han population and its clinical characteristics. Methods: Polymorphism of apolipoprotein C3 (-482C> T) in 300 NAFLD patients and 300 healthy controls was analyzed by polymerase chain reaction-restriction fragment length polymorphism and polyacrylamide gel electrophoresis. Logistic regression model was used to analyze the effect of polymorphism in this locus on NAFLD and its clinical features. Results: The frequency of apolipoprotein C3 (-482C> T) genotype was not significantly different between NAFLD patients and healthy controls (P> 0.05). T allele carriers of NAFLD patients are more likely to have insulin resistance, hypertension, hypertriglyceridemia, or lower HDL cholesterol than wild homozygotes CC. CONCLUSION: Polymorphism of apolipoprotein C3 (-482C> T) is not associated with susceptibility to NAFLD in Han Chinese, but carriers of T allele increase the risk of metabolic abnormalities in patients with NAFLD.