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The study on the coupling relationship and hydrology mechanism between ecosystem and hydrological process in a basin has re cently become the international research frontier in hydrology. Runoff separation is still an important subject and possibly cutting edge process in hydrology. This paper summarizes the progress of national and international research, and comments on the ad vantages and disadvantages of recent, diverse base flow separation methods. This paper also presents research on hydrological process and eco-hydrological function in different landscape zones, combining isotopic technology with hydrochemical methods. Based on the runoff separation of different water bodies, this paper probes into the coupling relationship and hydrology mecha nism between ecosystem pattern and eco-hydrological process, and makes analysis on water conservation, regulation and storage mechanism, and eco-hydrological function in different landscape zones. This report also examines future trends in research on hy drological process and eco-hydrological function in mountainous areas.