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目的探讨弥漫性脑损伤(DBI)中细胞外信号调节激酶1/2(ERK1/2)信号传导通路激活的时空变化特征。方法参照Marmarou方法制作大鼠DBI模型,Western blot法检测损伤脑组织磷酸化ERK1/2(pERK1/2)蛋白的表达,免疫组织化学法检测pERK1/2阳性细胞在损伤脑组织不同区域的分布。结果大鼠DBI后脑组织pERK1/2表达显著增高,5 min即达峰值,随即下降,但至12～24 h又有同升,达到第二个峰值,并持续高水平表达至72h。DBI损伤后30min～3 h主要见胞浆阳性染色,损伤后24 h在胞核、胞浆均可见阳性染色,以胞核为主。pERK1/2免疫阳性细胞多见于脑皮质深层,其次为海马,室管膜和脉络丛也可见pERK1/2阳性染色。结论大鼠DBI后ERK1/2通路被迅速和持久激活,pERK1/2免疫阳性细胞多见于脑皮质深层,也可见于海马、室管膜和脉络丛。
Objective To investigate the spatio-temporal changes of extracellular signal-regulated kinase 1/2 (ERK1 / 2) signaling pathway in diffuse brain injury (DBI). Methods The rat model of DBI was established by Marmarou method. The protein expression of phosphorylated ERK1 / 2 (pERK1 / 2) was detected by Western blot and the distribution of pERK1 / 2 positive cells in different regions of brain tissue was detected by immunohistochemistry. Results The expression of pERK1 / 2 increased significantly after DBI in rats, reaching the peak at 5 min and then decreasing. However, the level of pERK1 / 2 increased again after 12 to 24 h, reached the second peak and remained high continuously for 72 h. After 30min ~ 3h after DBI injury, cytoplasm positive staining was observed mainly in the nucleus and cytoplasm at 24 hours after injury. pERK1 / 2 immunopositive cells more common in the deep cerebral cortex, followed by the hippocampus, ependyme and choroid pERK1 / 2 positive staining can be seen. Conclusion The ERK1 / 2 pathway is rapidly and permanently activated after DBI in rats. The expression of pERK1 / 2 immunopositive cells is mainly found in the deep cerebral cortex and also in hippocampus, ependymal and choroid plexus.