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目的 利用线粒体DNA(m t DNA)上细胞色素氧化酶辅酶 和 (CO 、CO )中2 78bp和6 35 bp基因序列对嗜尸性苍蝇及其幼虫、卵进行种属鉴定。方法 随机采集放置在呼和浩特地区室外草地兔尸体和甘肃敦煌戈壁滩猪尸体上的嗜尸性苍蝇、幼虫、苍蝇腹中的卵,提取苍蝇m t DNA进行PCR扩增;并进行扩增结果检测;PCR胶回收纯化、测序并进行序列分析和构建系统发育树。结果 上述嗜尸性苍蝇mt DNA上CO 和(或) CO 基因序列在双翅目嗜尸性苍蝇的种内基因差异均数小于1% ,种间差异均数大于3% ,成虫与幼虫、卵之间几乎没有明显差异,表明在嗜尸性苍蝇种内和种间序列差异均数百分比范围内无重叠,并由此根据CO 和(或) CO 序列差异判断两个个体是否同种。结论 mt DNA上CO 和(或) CO 序列分析均能有效地对嗜尸性苍蝇及其幼虫、卵进行种属鉴定,CO +CO 和CO 的检验效能要高于CO 。
OBJECTIVE: To identify the species of sarcophagous flies, their larvae and eggs using the cytochrome oxidase coenzyme (COB) and mitochondrial DNA (COB) 2 78bp and 6 35 bp in mitochondrial DNA (m t DNA). Methods Randomly collected the eggs of the dead flies, larvae and flies belly of outdoor grass carcasses of the grassland rabbits in Hohhot and Gobi deserts in Gobi province, and extracted mt DNA of flies for PCR amplification. Gums were recovered for purification, sequenced and sequence analyzed and phylogenetic trees constructed. Results The average number of CO and / or CO gene sequences in the mtDNA of the zombie flies was less than 1% in the inbred flies of Diptera, and the mean number of species differences was more than 3%. The adults and larvae, the eggs There was almost no significant difference between the two species indicating that there was no overlap within the percentages of metacergic fly species and within the percentage differences in interspecific sequences and thus whether the two individuals were homozygous for differences in CO and / or CO sequences. Conclusion CO and / or CO sequence analysis on mt DNA can effectively identify species of sarcophagous flies and their larvae and eggs, and their CO + and CO contents are higher than that of CO.