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本研究利用CO_2培养箱内48孔板振荡光照培养专利设计的微藻高通量培养体系,结合细胞组学技术,系统评价了氮源和盐度梯度下胶球藻C-169细胞的生长速率、生物量浓度和油脂积累能力。结果表明,该培养体系的均一性和稳定性良好,可实现微藻的高通量及多处理培养。富氮培养基可显著提高胶球藻C-169的细胞生长速率和生物量浓度,缺氮培养基显著驱动了细胞积累中性脂。在含1/4氮的培养基中藻细胞的生物量浓度是4倍氮培养基中的25%,而中性脂含量(平均荧光强度表示)则高达后者的16倍以上。在0~15‰盐度范围内胶球藻C-169的生长状况良好且无显著差异(p>0.05),15‰以上盐度藻细胞生长受到明显抑制。10‰盐度下中性脂含量(以平均荧光强度表示)显著高于所有其他组(p<0.05),说明该盐度有利于驱动细胞积累中性脂。
In this study, we designed a micro-algae high-throughput culture system based on a 48-well plate oscillating light culture in a CO 2 incubator. Combined with the technology of cell assembly, we systematically evaluated the growth rate of C-169 cells under different nitrogen source and salinity gradients , Biomass concentration and oil accumulation ability. The results show that the culture system has good homogeneity and stability, and can achieve high-throughput micro-algae culture and multi-treatment. Nitrogen-rich medium significantly increased the cell growth rate and biomass concentration of C-169, and nitrogen-deficient medium significantly stimulated the cells to accumulate neutral lipids. The biomass concentration of algal cells in 1/4 ¼ nitrogen-containing medium was 25% of 4-fold nitrogen medium, whereas the neutral lipid content (mean fluorescence intensity) was more than 16 times higher than the latter. The growth of C-169 in the 0 ~ 15 ‰ salinity range was good and there was no significant difference (p> 0.05). The growth of algal cells above 15 ‰ was significantly inhibited. The neutral lipid content (expressed as mean fluorescence intensity) at 10 ‰ salinity was significantly higher than all other groups (p <0.05), indicating that salinity favors the accumulation of neutral lipids in cells.