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目的探讨microRNA-196a2 T>C基因多态性与肝硬化发生的相关性。方法采用聚合酶链反应和连接酶检测反应法(PCR-LDR)检测110例肝硬化患者及391例健康对照者microRNA-196a2 T>C基因多态性,运用SPSS 10.0软件对结果进行统计学分析,并分别对男性及女性个体microRNA-196a2 T>C基因多态性进行分析。结果肝硬化组与对照组都是以CT基因型为主,CC基因型及等位基因C在肝硬化组的分布频率(0.191,0.423)低于健康对照组(0.235,0.487),但无统计学差异(P>0.05);该位点基因型及等位基因在男性或女性个体中的分布趋势与整体趋势相似,相关差异也无统计学意义(P>0.05)。结论在中国汉族人群中,microRNA-196a2 T>C基因多态性与肝硬化的发生并没有明显相关性。
Objective To investigate the relationship between microRNA-196a2 T> C gene polymorphism and the occurrence of liver cirrhosis. Methods The polymorphism of microRNA-196a2 T> C in 110 cirrhotic patients and 391 healthy controls was detected by polymerase chain reaction and ligase assay (PCR-LDR). SPSS 10.0 software was used to analyze the results , And analyzed the polymorphism of microRNA-196a2 T> C gene in males and females respectively. Results The genotypes of CT were predominant in both cirrhosis and control groups. The distribution frequencies of CC genotype and allele C in cirrhosis group (0.191,0.423) were lower than those in healthy control group (0.235,0.487) (P> 0.05). The distribution trend of genotypes and alleles of this locus in the male or female individuals was similar to the overall trend, and there was no significant difference (P> 0.05). Conclusion There is no significant correlation between the polymorphism of microRNA-196a2 T> C and the occurrence of cirrhosis in Chinese Han population.