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目的:模拟人类Ⅱ型糖尿病的发病进程及代谢特征,建立实验性Ⅱ型糖尿病动物模型,并对该模型的糖代谢特征进行客观评价。方法:采用雄性SD大鼠以高脂高糖饲料喂养5周后,一次性腹腔注射小剂量链脲霉素(STZ,30mg/kg)。72h后检测空腹血糖(FBG)、口服糖耐量(OGTT)和胰岛素耐量(ITT)。选取空腹血糖7.0~25.0 mmol/L之间为成功模型标准,模型组大鼠继续喂养高脂高糖饲料4周,测定大鼠每日食物消耗量及饮水量,并于四周后检测糖代谢相关指标。结果:与正常对照组比较,糖尿病模型组大鼠体重减轻(P<0.05),饮食及饮水量显著增加,模型大鼠空腹血糖(FBG)、糖化血红蛋白(HbA lc)、血清胰岛素(FINS)、口服糖耐量(OGTT)与胰岛素耐量(ITT)曲线下面积显著高于正常对照组(P<0.01),同时表现胰岛素抵抗特征。结论:该模型具有临床Ⅱ型糖尿病患者糖代谢紊乱及胰岛素抵抗特征,与人类Ⅱ型糖尿病临床表现极为相似。
OBJECTIVE: To simulate the pathogenesis and metabolic characteristics of human type II diabetes mellitus, to establish an experimental animal model of type 2 diabetes mellitus, and to evaluate the objective of the glycometabolic characteristics of this model. Methods: Male SD rats were fed with high-fat and high-sucrose diet for 5 weeks, and once a small dose of streptozotocin (STZ, 30 mg / kg) was injected intraperitoneally. Fasting blood glucose (FBG), oral glucose tolerance (OGTT) and insulin tolerance (ITT) were measured after 72 hours. Select fasting blood glucose 7.0 ~ 25.0 mmol / L between the successful model standard, the model group rats continue to feed high-fat and high-sugar diet for 4 weeks, the rats daily food consumption and water intake, and after four weeks to detect glucose metabolism related index. Results: Compared with the normal control group, the body weight of diabetic rats in model group was significantly decreased (P <0.05) and the diet and water intake were significantly increased. The fasting blood glucose (FBG), HbAlc, FINS, Oral glucose tolerance (OGTT) and insulin tolerance (ITT) area under the curve was significantly higher than the normal control group (P <0.01), while showing the characteristics of insulin resistance. Conclusion: This model has the features of glucose metabolism disorder and insulin resistance in clinical type Ⅱ diabetic patients, which is very similar to the clinical manifestations of human type Ⅱ diabetes mellitus.