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在建立了活体研究苹果果实糖卸载体系———“卸载穴技术”的基础上，对金冠苹果果实糖迅速积累期糖卸载的生理机制进行了研究。１ｍｍｏｌ／ＬＰＣＭＢＳ（对氯高汞苯磺酸）、１ｍｍｏｌ／ＬＤＮＰ（２，４－二硝基酚）、１ｍｍｏｌ／ＬＥＢ（曙红Ｂ）和８００ｍｍｏｌ／ＬＢｅｔａｉｎｅ（甜菜碱）明显抑制苹果果实糖的卸载，１０－５ｍｏｌ／ＬＡＢＡ（脱落酸）、５ｍｍｏｌ／ＬＥＧＴＡ（乙二醇双乙胺醚－Ｎ，Ｎ，Ｎ′，Ｎ′－乙酸）和５０ｍｍｏｌ／ＬＮＯ－３明显促进苹果果实糖的卸载。由此可见，苹果果实糖卸载过程中，共质体途径和质外体途径都存在；在苹果果实果肉细胞的质膜上，存在糖的载体；糖跨苹果果实果肉细胞的质膜和液泡膜的运输是需要能量的主动过程。
On the basis of the in-vivo study on apple fruit sugar unloading system --- “unloading technique”, the physiological mechanism of sugar unloading during the rapid accumulation of sugar in Golden Delicious apple fruit was studied. 1 mmol / L PCMBS, 1 mmol / L LDNP, 1 mmol / LEB and 800 mmol / L betaine significantly inhibited apple fruit sugar unloading , 10-5mol / LABA (abscisic acid), 5mmol / L EGTA (ethylene glycol bisethylamine-N, N, N’-N’- acetic acid) and 50mmol / LNO-3 significantly promoted the unloading of apple fruit sugar. Thus, both the plastid pathway and the apoplast pathway exist in the unloading process of apple fruit sugar. On the plasma membrane of apple fruit pulp cells, there is a carrier of sugar. The plasma membrane and tonoplast of sugar fruit cross the apple fruit Transport is an active process that requires energy.