论文部分内容阅读
目的对本中心送检的免疫性血小板输注无效血液病患者进行血小板抗体特异性检测与分析。方法采用固相凝集法对多次输注血小板并发生血小板输注无效(PTR)的血液病患者进行血小板抗体筛检,对其中血小板抗体阳性患者采用PAKPLUS试剂盒进行血小板抗体鉴定。结果共筛选出血小板抗体阳性标本115例,其中抗-HLA-Ⅰ类69例(60.00%),抗-HPA 3例(2.61%),抗-HLA-Ⅰ类和抗-HPA 43例(37.39%);46例抗-HPA阳性标本中,单一血小板膜糖蛋白抗体22例(47.83%),其中抗GPⅡb/Ⅲa 6例(13.04%),抗GPⅠa/Ⅱa 10例(21.74%),抗GPⅣ6例(13.04%),多个血小板膜糖蛋白抗体24例(52.17%)。结论抗-HLA-Ⅰ和抗-HPA是导致血液病患者免疫性PTR的主要因素,血小板抗体检测可为临床血小板输注策略提供依据,提高血小板输注效果。
Objective To detect and analyze the specific antibody of platelet in patients with ineffective hematological diseases of immunological platelet transfusion. Methods Platelet antibody screening was performed on patients with hematological diseases with multiple platelet transfusion and ineffective platelet transfusion (PTR) by solid-phase agglutination. PAKPLUS kit was used to identify platelet antibodies in patients with positive platelet antibodies. Results A total of 115 cases of platelet antibody positive samples were screened, of which 69 cases were anti-HLA-Ⅰ (60.00%), 3 cases were anti-HPA (2.61%), 43 cases were anti-HLA- ). Among 46 anti-HPA positive samples, 22 cases (47.83%) had single platelet glycoprotein antibody, including 6 cases (13.04%) of GPⅡb / Ⅲa, 10 cases of GPⅠa / Ⅱa (13.04%), multiple platelet glycoprotein antibodies in 24 cases (52.17%). Conclusion Anti-HLA-Ⅰ and anti-HPA are the main factors leading to immune PTR in patients with hematological diseases. Detection of platelet antibodies may provide a basis for clinical platelet transfusion strategy and improve platelet transfusion.